4TMX
Translation initiation factor eIF5B (517-858) mutant D533N from C. thermophilum, bound to GTP and sodium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.530, 116.820, 66.490 |
| Unit cell angles | 90.00, 101.02, 90.00 |
Refinement procedure
| Resolution | 46.430 - 1.500 |
| R-factor | 0.1564 |
| Rwork | 0.155 |
| R-free | 0.18160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ncn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.619 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (2.5.2) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1690)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.430 | 1.590 | |
| High resolution limit [Å] | 1.500 | 6.710 | 1.500 |
| Rmerge | 0.012 | ||
| Rmeas | 0.029 | 0.014 | 0.598 |
| Total number of observations | 542891 | ||
| Number of reflections | 131458 | 1523 | 9467 |
| <I/σ(I)> | 24.4 | 119.58 | 2.9 |
| Completeness [%] | 99.2 | 99.5 | 98.2 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 293 | HEPES, PEG 4000, NaOAc |
