4S3N
Crystal structure of human OAS3 domain I in complex with dsRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-09-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.700, 104.700, 63.800 |
| Unit cell angles | 90.00, 97.80, 90.00 |
Refinement procedure
| Resolution | 35.479 - 2.000 |
| R-factor | 0.1833 |
| Rwork | 0.182 |
| R-free | 0.21130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ig8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.977 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.820 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 42802 | |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 200 mM trisodium citrate, 150 mM NaCl, 12% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
