4S0O
Crystal Structure of the Autoinhibited Dimer of Pro-apoptotic BAX (I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.657, 40.276, 65.303 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 19.150 - 1.900 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f16 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.856 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.600 |
| Number of reflections | 24766 | |
| <I/σ(I)> | 21.7 | 2.3 |
| Completeness [%] | 95.0 | 98 |
| Redundancy | 4.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 1.6 M AMMONIUM SULFATE, 0.1 M BIS-TRIS , PH 6.2, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
