4RZM
Crystal structure of the Lsd19-lasalocid A complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 140.140, 140.140, 198.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.160 - 2.330 |
| R-factor | 0.17887 |
| Rwork | 0.177 |
| R-free | 0.20671 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rga |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.838 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.160 | 2.390 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.082 | 0.979 |
| Number of reflections | 42332 | |
| <I/σ(I)> | 16.95 | 2.45 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291.15 | 1.65 M sodium formate, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
