4RXZ
Crystal Structure of MDMX phosporylated Tyr99 in complex with a 12-mer peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9753 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.613, 41.430, 45.618 |
| Unit cell angles | 103.32, 103.67, 102.44 |
Refinement procedure
| Resolution | 42.330 - 1.550 |
| R-factor | 0.18249 |
| Rwork | 0.180 |
| R-free | 0.22034 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eqy |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.964 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.330 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.059 | 0.485 |
| Number of reflections | 25745 | |
| <I/σ(I)> | 16.14 | 1.1 |
| Completeness [%] | 93.0 | 87.4 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 12% isopropanol, 0.1M MES pH 6.5 and 10% PEG5000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
