4RHW

Crystal structure of Apaf-1 CARD and caspase-9 CARD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-16
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	64.531, 92.520, 68.031
Unit cell angles	90.00, 109.12, 90.00

Refinement procedure

Resolution	23.774 - 2.100
R-factor	0.2113
Rwork	0.210
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ygs
RMSD bond length	0.013
RMSD bond angle	1.123
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_596))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	43908
Completeness [%]	99.7	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	13% PEG 3000, 0.2M ammonium sulfate, 0.1M MES (pH5.5), VAPOR DIFFUSION, HANGING DROP, temperature 291K