4R7P

Human UDP-glucose pyrophosphorylase isoform 1 in complex with UDP-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-21
Detector	ADSC QUANTUM 210
Spacegroup name	P 31 2 1
Unit cell lengths	138.970, 138.970, 311.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.620 - 3.350
R-factor	0.202
Rwork	0.199
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.101
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.623
High resolution limit [Å]	3.300
Number of reflections	58213
Completeness [%]	99.7
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	281.15	The protein sample contained 10.3 mg/ml of hUGP, 50 mM HEPES pH 7.5, 5 mM MgCl2, 1 mM EDTA, 20% (w/v) sucrose and 4 mM UDP-Glc. 1 l of the hUGP UDP-Glc complex was mixed 1:1 with the reservoir solution containing 100 mM sodium acetate buffer pH 4.8, 520 mM zinc acetate, 6% (w/v) aminocaproic acid and 75 mM ammonium sulfate. , VAPOR DIFFUSION, SITTING DROP, temperature 281.15K