4R0L
Anti-canine CD28 antibody, 1C6, bound canine CD28
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.27 |
| Spacegroup name | P 31 |
| Unit cell lengths | 86.783, 86.783, 205.804 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.460 - 3.300 |
| R-factor | 0.25792 |
| Rwork | 0.256 |
| R-free | 0.29283 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.391 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.065 | 0.484 |
| Number of reflections | 25360 | |
| <I/σ(I)> | 15.1 | 2.3 |
| Completeness [%] | 96.9 | 97.5 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.9M AmSO4, 0.1M Cesium sulfate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
