4R0E
Crystal Structure of the Poliovirus RNA-Dependent RNA Polymerase Low-Fidelity Mutant 3Dpol H273R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-02-26 |
Detector | RIGAKU |
Wavelength(s) | 0.9 |
Spacegroup name | P 65 |
Unit cell lengths | 126.440, 126.440, 113.246 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.870 - 3.000 |
R-factor | 0.18763 |
Rwork | 0.186 |
R-free | 0.21767 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ra6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.467 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.067 | 0.313 |
Number of reflections | 19450 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 5.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | Mixed equal volumes of protein 10mg/ml in buffer (5mM Tris pH 7.5, 200mM NaCl, 0.1mM EDTA, 2mM DTT) and reservoir solution (2M Na Acetate, 0.1 M Na Cacodylate pH 6.8) prior to vapor diffusion, VAPOR DIFFUSION, temperature 293K |