4QTT

Structure of S. cerevisiae Bud23-Trm112 complex involved in formation of m7G1575 on 18S rRNA (apo-form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-14
Detector	PSI PILATUS 6M
Wavelength(s)	0.9801
Spacegroup name	P 1 21 1
Unit cell lengths	74.420, 53.880, 83.760
Unit cell angles	90.00, 95.32, 90.00

Refinement procedure

Resolution	37.050 - 2.000
R-factor	0.1937
Rwork	0.191
R-free	0.24200
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.044
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.000	2.000
Number of reflections	44594
<I/σ(I)>	18.3
Completeness [%]	96.5	96.5
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
2