4QTI
Crystal structure of human uPAR in complex with anti-uPAR Fab 8B12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 45.554, 95.403, 189.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.770 - 3.000 |
| R-factor | 0.25196 |
| Rwork | 0.250 |
| R-free | 0.29625 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fd6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.027 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.770 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 16359 | |
| Completeness [%] | 94.4 | 95.8 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 48% MPD, 100mM Tris, 100mM NaCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
