4QQZ

Crystal structure of T. fusca Cas3-AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	86.917, 222.064, 124.898
Unit cell angles	90.00, 104.29, 90.00

Refinement procedure

Resolution	50.110 - 2.930
R-factor	0.183
Rwork	0.182
R-free	0.23400
RMSD bond length	0.015
RMSD bond angle	1.410
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.080
High resolution limit [Å]	2.930	2.930
Rmerge	0.082	0.390
Number of reflections	97476
<I/σ(I)>	9.4	3
Completeness [%]	99.3	99.9
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	294	100 mM MES, pH 6.5, and 5% 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K
1		6.5	294	100 mM MES, pH 6.5, and 5% 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K