4QKX

Structure of beta2 adrenoceptor bound to a covalent agonist and an engineered nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	78
Detector technology	CCD
Collection date	2013-06-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	49.558, 67.211, 301.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.260 - 3.300
R-factor	0.2401
Rwork	0.236
R-free	0.27950
RMSD bond length	0.005
RMSD bond angle	0.634
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1241)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.300
High resolution limit [Å]	3.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7.8	293	24-27 % PEG400, 100 mM Tris pH 7.8 to pH 8.4, 5-10 mM Sodium formate, lipidic cubic phase, temperature 293K