4QKB

Crystal structure of seleno-methionine labelled human ALKBH7 in complex with alpha-ketoglutarate and Mn(II)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	130
Detector technology	CCD
Collection date	2013-05-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97929, 0.97943
Spacegroup name	P 1 21 1
Unit cell lengths	66.737, 82.759, 66.724
Unit cell angles	90.00, 119.95, 90.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.1589
Rwork	0.158
R-free	0.18103
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.152
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	17632
Completeness [%]	90.5	51

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	277	PEG pH6, EVAPORATION, temperature 277K