4PR9
Human Vinculin (residues 891-1066) in complex with PIP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-12 |
Detector | RAYONIX MX300HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 102.579, 102.579, 190.772 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.170 - 3.200 |
R-factor | 0.2062 |
Rwork | 0.204 |
R-free | 0.25010 |
Structure solution method | MIR |
Starting model (for MR) | 1rke |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | MOLREP |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.836 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.053 | |
Number of reflections | 19218 | |
Completeness [%] | 99.7 | |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 12% PEG3350, 2% tryptone, 0.05 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |