4PO6
Crystal structure of the human TYK2 FERM and SH2 domains with an IFNAR1 intracellular peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-14 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.768, 52.422, 70.163 |
| Unit cell angles | 90.00, 108.09, 90.00 |
Refinement procedure
| Resolution | 38.550 - 1.990 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| Starting model (for MR) | SAD MODEL |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.550 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.049 | 0.615 |
| Number of reflections | 42667 | |
| <I/σ(I)> | 15.5 | 2.2 |
| Completeness [%] | 96.4 | 92.2 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 0.1 M Tris HCl pH 7.0, 0.2 M MgCl2, and 8-12% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
