4PNI
Bovine G protein-coupled receptor kinase 1 in complex with GSK2163632A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 45.485, 66.566, 205.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.670 - 1.850 |
| R-factor | 0.19054 |
| Rwork | 0.188 |
| R-free | 0.23024 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.004 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 51503 | |
| <I/σ(I)> | 90 | 1.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.3 | 19.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | MES, PEG 3350, NaCl |
