4PLE
Human Nuclear Receptor Liver Receptor Homologue-1, LRH-1, Bound to an E. Coli Phospholipid and a Fragment of TIF-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.947, 76.885, 108.482 |
| Unit cell angles | 90.00, 95.46, 90.00 |
Refinement procedure
| Resolution | 46.620 - 1.752 |
| R-factor | 0.2145 |
| Rwork | 0.214 |
| R-free | 0.23620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yok |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.707 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.620 | 1.814 |
| High resolution limit [Å] | 1.752 | 1.752 |
| Rmerge | 0.066 | 0.309 |
| Total number of observations | 365113 | |
| Number of reflections | 100330 | |
| <I/σ(I)> | 12.77 | 3.2 |
| Completeness [%] | 92.5 | 62.76 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.7-1 M di-Sodium Malonate, 0.1 M HEPES pH 7.4, 0.5% Jeffamine ED-2001 |
