4PKD
U1-70k in complex with U1 snRNA stem-loops 1 and U1-A RRM in complex with stem-loop 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.220, 66.600, 93.730 |
| Unit cell angles | 90.00, 110.95, 90.00 |
Refinement procedure
| Resolution | 87.000 - 2.500 |
| R-factor | 0.19992 |
| Rwork | 0.197 |
| R-free | 0.25791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1urn |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.910 |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.540 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.076 | 0.350 |
| Number of reflections | 15967 | |
| <I/σ(I)> | 11 | 3.2 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 40% MPD, 0.15 M sodium chloride, 0.1 M sodium acetate, pH 4.2 |
