4PEH

Dbr1 in complex with synthetic linear RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	73.021, 141.936, 212.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.033 - 2.100
R-factor	0.2056
Rwork	0.203
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.907
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.980	2.210
High resolution limit [Å]	2.100	2.100
Rmerge		0.521
Number of reflections	129196
<I/σ(I)>	11	2.8
Completeness [%]	99.5	99.4
Redundancy	4.8	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.2 M lithium sulfate, 0.1 M bis-tris, 25% PEG 3350, 8.5 % glycerol, 3.0 mM manganese sulfate, 1.45 mM AK65