4PAN

A conserved phenylalanine as relay between the 5 helix and the GDP binding region of heterotrimeric G protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978
Spacegroup name	I 4
Unit cell lengths	121.533, 121.533, 68.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.1872
Rwork	0.182
R-free	0.23220
RMSD bond length	0.008
RMSD bond angle	1.011
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400
Number of reflections	19424
<I/σ(I)>	19.3
Completeness [%]	99.2
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	(NH4)2SO3, Sodium acetate