4P7I

Crystal structure of the Merlin FERM/DCAF1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 31 2 1
Unit cell lengths	97.104, 97.104, 224.339
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.470 - 2.600
R-factor	0.2258
Rwork	0.224
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1isn
RMSD bond length	0.003
RMSD bond angle	0.674
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.079	0.048	0.925
Total number of observations	175045
Number of reflections	37859
<I/σ(I)>	13
Completeness [%]	98.2	78	99.9
Redundancy	4.6	3.7	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289	20% Isopropanol and 5% PEG8000