4P3W
Crystal structure of the human filamin A Ig-like domains 20-21 in complex with migfilin peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | H 3 |
| Unit cell lengths | 88.154, 88.154, 394.709 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.860 - 2.000 |
| R-factor | 0.2016 |
| Rwork | 0.200 |
| R-free | 0.22590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2brq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.996 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.360 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.053 |
| Rmeas | 0.062 |
| Total number of observations | 354375 |
| Number of reflections | 103427 |
| <I/σ(I)> | 14.07 |
| Completeness [%] | 97.9 |
| Redundancy | 3.426 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1 M MES pH 6, 1.9 M (NH4)2SO4, 0.1 M (CH3CO2)3Pr |
