4OZT
crystal structure of the ligand binding domains of the Bovicola ovis ecdysone receptor EcR/USP heterodimer (PonA crystal)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-28 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9536 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 134.000, 134.000, 95.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.830 - 2.700 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.418 |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.800 |
High resolution limit [Å] | 2.700 |
Number of reflections | 24458 |
<I/σ(I)> | 14.8 |
Completeness [%] | 100.0 |
Redundancy | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350 with either 0.2 M potassium acetate or 0.2 M sodium citrate |