4OYS
CRYSTAL STRUCTURE OF VPS34 IN COMPLEX WITH SAR405.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 89.810, 145.700, 61.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.850 - 2.900 |
| Rwork | 0.180 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in-house |
| Data reduction software | PROCESS |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.910 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 18671 | |
| <I/σ(I)> | 8.8 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.9 M ammonium sulfate, 100 mM Tris pH 8.5 |
