4OL8
Ty3 reverse transcriptase bound to DNA/RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 320.700, 75.062, 108.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.898 - 3.100 |
| R-factor | 0.2305 |
| Rwork | 0.227 |
| R-free | 0.29560 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.069 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.898 | 3.290 |
| High resolution limit [Å] | 2.999 | 2.999 |
| Rmerge | 0.102 | 0.847 |
| Number of reflections | 53285 | |
| <I/σ(I)> | 11.9 | 2.1 |
| Completeness [%] | 99.6 | 98.4 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1 M Tris, 0.2 M ammonium sulfate, 17% PEG3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
