4OKN

Crystal structure of human muscle L-lactate dehydrogenase, ternary complex with NADH and oxalate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	65.550, 159.620, 266.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.000 - 2.100
R-factor	0.20777
Rwork	0.206
R-free	0.24504
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.266
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.000	34.000	2.150
High resolution limit [Å]	2.100	9.390	2.100
Number of reflections	163531
<I/σ(I)>	14.3
Completeness [%]	99.9	95	100
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	18% w/v PEG3350, 50 mM HEPES, pH 6.8, Silver Bullets Bio Screen 1 reagent A1 (Hampton Research), VAPOR DIFFUSION, HANGING DROP, temperature 293K