4OC7
Retinoic acid receptor alpha in complex with (E)-3-(3'-allyl-6-hydroxy-[1,1'-biphenyl]-3-yl)acrylic acid and a fragment of the coactivator TIF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.554, 67.554, 110.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.768 - 2.500 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.750 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.770 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 9327 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 25.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M Na HEPES pH 7.5, 20% PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
