4O61
Structure of human ALKBH5 crystallized in the presence of citrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-22 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.471, 57.172, 78.644 |
| Unit cell angles | 90.00, 102.23, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.1767 |
| Rwork | 0.174 |
| R-free | 0.23030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h8r |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.457 |
| Data scaling software | Aimless (0.2.14) |
| Phasing software | BALBES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.430 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.129 | 0.983 |
| Total number of observations | 15835 | |
| Number of reflections | 34700 | |
| <I/σ(I)> | 13.7 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 20% PEG3350, 0.2 M diammonium citrate, temperature 291K |
