4O2B
Tubulin-Colchicine complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-03 |
Detector | PSI PILATUS 2M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.310, 157.010, 180.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.700 - 2.300 |
R-factor | 0.183 |
Rwork | 0.182 |
R-free | 0.21300 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 4i4t |
RMSD bond length | 0.002 |
RMSD bond angle | 0.651 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.700 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.115 | 1.943 |
Number of reflections | 149841 | |
<I/σ(I)> | 21.3 | 1.7 |
Completeness [%] | 99.7 | 97.9 |
Redundancy | 13.6 | 12.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.7 | 293 | 6% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazole , pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |