4O1P
Crystal Structure of RNase L in complex with 2-5A and AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.197, 267.392, 110.210 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 59.200 - 2.500 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.23107 |
| Structure solution method | MR-SAD |
| Starting model (for MR) | 4g8k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.231 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.200 | 2.569 |
| High resolution limit [Å] | 2.500 | 2.504 |
| Rmerge | 0.104 | 0.526 |
| Number of reflections | 106347 | |
| <I/σ(I)> | 6.8 | 1.6 |
| Completeness [%] | 95.7 | 88.4 |
| Redundancy | 3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 5 mM MgCl2, 5 mM DTT, 18% PEG2000, 100 mM NaCl, and 100 mM SPG buffer, pH 7. , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
