4O1O
Crystal Structure of RNase L in complex with 2-5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.792, 111.968, 268.465 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 49.700 - 3.270 |
| R-factor | 0.23225 |
| Rwork | 0.230 |
| R-free | 0.28851 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o1p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.394 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.700 | 3.352 |
| High resolution limit [Å] | 3.250 | 3.267 |
| Number of reflections | 51209 | |
| Completeness [%] | 94.1 | 92.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 5 mM DTT, 18% PEG2000, 100 mM NaCl, and 100 mM SPG buffer, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
