4NY0
Crystal structure of FERM domain of human focal adhesion kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115869 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.960, 152.500, 95.340 |
| Unit cell angles | 90.00, 93.62, 90.00 |
Refinement procedure
| Resolution | 95.150 - 2.800 |
| R-factor | 0.1952 |
| Rwork | 0.193 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.409 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 95.150 | 95.150 | 2.930 |
| High resolution limit [Å] | 2.800 | 5.600 | 2.800 |
| Rmerge | 0.047 | 0.018 | 0.651 |
| Number of reflections | 49012 | ||
| <I/σ(I)> | 22.2 | 1.4 | |
| Completeness [%] | 99.9 | 79.5 | |
| Redundancy | 2 | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 17% PEG 10000, 0.1M Ammonium Sulfate, 0.1M bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
