4NWT
Crystal structure of the anti-human NGF Fab APE1531
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2012-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97830 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.720, 64.360, 63.810 |
| Unit cell angles | 90.00, 108.67, 90.00 |
Refinement procedure
| Resolution | 38.640 - 1.750 |
| R-factor | 0.15194 |
| Rwork | 0.150 |
| R-free | 0.18121 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3QOT for VHVL 3SOB for CH1CL |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.988 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.640 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.048 | 0.604 |
| Number of reflections | 48714 | |
| <I/σ(I)> | 25.3 | 2.9 |
| Completeness [%] | 98.5 | 97.7 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 12% PEG 20000, 0.1M NaCl, 0.1M MES pH6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
