4NUB

Crystal structure of Escherichia coli ribosomal oxygenase YcfD

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-09
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97913, 0.97927, 0.91837
Spacegroup name	P 43 21 2
Unit cell lengths	75.731, 75.731, 210.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.926 - 2.700
R-factor	0.1806
Rwork	0.178
R-free	0.22830
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.218
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.700
Rmerge	0.070
Number of reflections	31993
<I/σ(I)>	11.5
Completeness [%]	99.9
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	3.6% PEG 4000, 30% Glycerol, 0.07M sodium acetate, 0.5 uL of 1/10 dilution of seed solution, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K