4NPL

Crystal structure of Zebrafish ALKBH5 in complex with alpha-ketoglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	180
Detector technology	CCD
Collection date	2013-08-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9676
Spacegroup name	P 21 21 21
Unit cell lengths	65.948, 68.595, 114.909
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.827 - 1.651
R-factor	0.2098
Rwork	0.209
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.140
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.650
Number of reflections	60317

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.2 M Sodium iodide, pH 7.0, 20% w/v Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K