4NNU

Distinct structural features of TFAM drive mitochondrial DNA packaging versus transcriptional activation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	I 2 2 2
Unit cell lengths	109.800, 114.380, 144.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.017 - 2.810
R-factor	0.2181
Rwork	0.214
R-free	0.26110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tq6
RMSD bond length	0.006
RMSD bond angle	1.363
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.870
High resolution limit [Å]	2.810
Rmerge	0.084
Number of reflections	22010
Completeness [%]	30.0
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	9% PEG1500 and 18% glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K