4NLC
Crystal structure of the catalytic core of RRP6 from Trypanosoma brucei, mutant C496S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.410, 93.410, 49.990 |
Unit cell angles | 90.00, 105.23, 90.00 |
Refinement procedure
Resolution | 48.230 - 2.150 |
R-factor | 0.173 |
Rwork | 0.171 |
R-free | 0.22080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nlb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 19025 | |
<I/σ(I)> | 9.1 | 1.6 |
Completeness [%] | 99.5 | 98.5 |
Redundancy | 3.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 25% PEG2000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |