4NIF

Heterodimeric structure of ERK2 and RSK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91961
Spacegroup name	P 1 21 1
Unit cell lengths	93.490, 87.850, 116.540
Unit cell angles	90.00, 108.22, 90.00

Refinement procedure

Resolution	60.630 - 2.150
R-factor	0.1605
Rwork	0.158
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2Y9Q(for chain B E) 2WNT(for chain A D).
RMSD bond length	0.007
RMSD bond angle	1.028
Refinement software	PHENIX ((phenix.refine: dev_1439))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.630	2.227
High resolution limit [Å]	2.150	2.150
Number of reflections	97338
Completeness [%]	99.7	99.85

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.25	296	0.1M MES, 15% PEG4000, 0.125M (NH4)2SO4, 2% Benzamidine, pH 6.25, VAPOR DIFFUSION, SITTING DROP, temperature 296K