4NCL

Crystal structure of eukaryotic translation initiation factor eIF5B (517-970) from Chaetomium thermophilum in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.826580
Spacegroup name	P 21 21 21
Unit cell lengths	66.900, 72.960, 199.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.865 - 2.115
R-factor	0.2157
Rwork	0.214
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 4N3N
RMSD bond length	0.005
RMSD bond angle	0.956
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			2.210
High resolution limit [Å]	2.120	50.000	2.120
Rmerge	0.056		0.631
Number of reflections	56555
<I/σ(I)>	17.79		2.97
Completeness [%]	99.7		99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15 % PEG 8000, 0.5 M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K