4NC9
Crystal structure of phosphatidyl mannosyltransferase PimA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0668 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 137.620, 137.620, 168.641 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.520 - 3.192 |
R-factor | 0.2141 |
Rwork | 0.212 |
R-free | 0.25620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gek |
RMSD bond length | 0.005 |
RMSD bond angle | 0.903 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.520 | 3.360 |
High resolution limit [Å] | 3.190 | 3.190 |
Rmerge | 0.061 | 0.512 |
Number of reflections | 31022 | |
Completeness [%] | 99.6 | 96.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 15% PEG 4000, 200 mM MgCl2, 100 mM Tris-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 8.0 |