4N76

Structure of Thermus thermophilus Argonaute bound to guide DNA and cleaved target DNA with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	110.918, 117.420, 160.457
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.372 - 2.890
R-factor	0.2215
Rwork	0.219
R-free	0.26870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n47
RMSD bond length	0.007
RMSD bond angle	1.107
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.890	2.890
Rmerge	0.160
Number of reflections	45264
Completeness [%]	95.5	82.2
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	306	2.8 M sodium acetate, 0.1 M Bis-Tris propane, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 306K