4N41

Structure of Thermus thermophilus Argonaute bound to guide DNA and 15-mer target DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	59.513, 101.699, 153.019
Unit cell angles	90.00, 93.52, 90.00

Refinement procedure

Resolution	45.524 - 2.248
R-factor	0.2248
Rwork	0.223
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f73
RMSD bond length	0.011
RMSD bond angle	1.274
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.300
High resolution limit [Å]	2.248	5.550	2.248
Rmerge	0.070	0.032	0.772
Number of reflections	86435
<I/σ(I)>	10.5
Completeness [%]	99.8	98.9	98.9
Redundancy	7.4	7.1	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	306	3.2 M sodium chloride, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 306K