4MZF

Crystal structure of human Spindlin1 bound to histone H3(K4me3-R8me2a) peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	77
Detector technology	CCD
Collection date	2012-07-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 21 21 2
Unit cell lengths	120.679, 43.309, 50.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.778 - 2.098
R-factor	0.205
Rwork	0.201
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.182
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.740	2.140
High resolution limit [Å]	2.098	2.650	2.098
Rmerge	0.091	0.179	0.592
Number of reflections	15029
<I/σ(I)>	28.47	12.56	3.74
Completeness [%]	94.0	94.9	90.5
Redundancy	4.6	4.4	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	20% PEG 8000, 20% PEG 400, 0.1M MgCl2, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K