4MXW

Structure of heterotrimeric lymphotoxin LTa1b2 bound to lymphotoxin beta receptor LTbR and anti-LTa Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-28
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.978
Spacegroup name	C 1 2 1
Unit cell lengths	211.201, 52.428, 253.241
Unit cell angles	90.00, 101.10, 90.00

Refinement procedure

Resolution	49.701 - 3.600
R-factor	0.234
Rwork	0.231
R-free	0.29600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1TNR AND 1FVE
RMSD bond length	0.013
RMSD bond angle	1.581
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.701
High resolution limit [Å]	3.600	3.600
Number of reflections	32265
Completeness [%]	92.8
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	292	15% PEG6000, 0.1 M sodium citrate, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 292K