4MHL

The crystal structure of human interleukin-11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 2
Unit cell lengths	118.862, 28.169, 41.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.810 - 2.090
R-factor	0.18855
Rwork	0.184
R-free	0.24155
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1alu
RMSD bond length	0.009
RMSD bond angle	1.389
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.810	2.150
High resolution limit [Å]	2.090	2.090
Rmerge	0.090	0.459
Number of reflections	8862
<I/σ(I)>	11.5	2.9
Completeness [%]	99.6	97.5
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	1.25 M ammonium sulfate, 0.2 M lithium sulfate, 0.1 M bis-tris propane, 10% formamide, 0.02% sodium azide, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K