4MH5

Crystal structure of the kainate receptor GluK3 ligand binding domain in complex with (S)-glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9184
Spacegroup name	P 41 2 2
Unit cell lengths	67.800, 67.800, 122.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.897 - 1.650
R-factor	0.1317
Rwork	0.129
R-free	0.18140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ycj
RMSD bond length	0.010
RMSD bond angle	1.271
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.897	27.897	1.740
High resolution limit [Å]	1.650	5.220	1.650
Rmerge		0.033	0.373
Number of reflections	35179
<I/σ(I)>	7	14.6	2
Completeness [%]	100.0	99.1	100
Redundancy	7.8	7	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	293	1.8 M sodium/potassium phosphate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K