4MGM

Crystal structure of the in vitro transcribed G. kaustophilus tRNA-Gly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-01
Detector	ADSC QUANTUM 210
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	161.233, 28.409, 132.939
Unit cell angles	90.00, 110.69, 90.00

Refinement procedure

Resolution	33.169 - 3.200
R-factor	0.2485
Rwork	0.248
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l0u
RMSD bond length	0.001
RMSD bond angle	0.403
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.260
High resolution limit [Å]	3.200	8.640	3.200
Rmerge	0.158	0.063	0.324
Number of reflections	8419
<I/σ(I)>	5.5	24.7	3.14
Completeness [%]	82.8	99.8	46.2
Redundancy	5.6	6.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	75mM NaCl, 2mM CoCl2, 50mM sodium cacodylate, 0.5mM spermine, 30% w/v 1,6-Hexanediol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K