4MBE
Sac3:Sus1:Cdc31:Nup1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.270, 62.399, 124.601 |
| Unit cell angles | 90.00, 98.61, 90.00 |
Refinement procedure
| Resolution | 43.800 - 2.612 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fwb |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.831 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.800 | 2.730 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 23841 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
