3FWB

Sac3:Sus1:Cdc31 complex

Summary for 3FWB

• Entry DOI: 10.2210/pdb3fwb/pdb
• Related: 3FWC
• Descriptor: Cell division control protein 31, Nuclear mRNA export protein SAC3, Protein SUS1, ... (4 entities in total)
• Functional Keywords: gene gating, complex, cell cycle, cell division, mitosis, mrna transport, nuclear pore complex, nucleus, phosphoprotein, protein transport, translocation, transport, transcription, transcription regulation
• Biological source: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast); More
• Cellular location: Nucleus, nuclear pore complex: P06704; Nucleus envelope: P46674; Nucleus, nucleoplasm: Q6WNK7
• Total number of polymer chains: 3
• Total formula weight: 36636.24

Authors

Stewart, M.,Jani, D. (deposition date: 2009-01-17, release date: 2009-04-14, Last modification date: 2024-04-03)

Primary citation

Jani, D.,Lutz, S.,Marshall, N.J.,Fischer, T.,Kohler, A.,Ellisdon, A.M.,Hurt, E.,Stewart, M.
Sus1, Cdc31, and the Sac3 CID region form a conserved interaction platform that promotes nuclear pore association and mRNA export.
Mol.Cell, 33:727-737, 2009

PubMed Abstract: The yeast Sac3:Cdc31:Sus1:Thp1 (TREX-2) complex facilitates the repositioning and association of actively transcribing genes with nuclear pores (NPCs)-"gene gating"-that is central to integrating transcription, processing, and mRNA nuclear export. We present here the crystal structure of Sus1 and Cdc31 bound to a central region of Sac3 (the CID domain) that is crucial for its function. Sac3(CID) forms a long, gently undulating alpha helix around which one Cdc31 and two Sus1 chains are wrapped. Sus1 has an articulated helical hairpin fold that facilitates its wrapping around Sac3. In vivo studies using engineered mutations that selectively disrupted binding of individual chains to Sac3 indicated that Sus1 and Cdc31 function synergistically to promote NPC association of TREX-2 and mRNA nuclear export. These data indicate Sac3(CID) provides a scaffold within TREX-2 to integrate interactions between protein complexes to facilitate the coupling of transcription and mRNA export during gene expression.

PubMed: 19328066
DOI: 10.1016/j.molcel.2009.01.033

Experimental method

X-RAY DIFFRACTION (2.5 Å)